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Genomic differences between individuals can change the physical organization of RNA transcripts

The information contained within a messenger RNA (mRNA) transcript goes beyond the protein recipe embedded in its sequence. mRNAs consist of single strands of nucleotides that can pair with each other in the same way as double-stranded DNA molecules. The resulting ‘secondary structures’ help determine when and how the encoded protein gets produced.

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3D Animations – Transcription & Translation: RNA Splicing :: DNA Learning Center

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3D Animations – Transcription & Translation: Transcription (Advanced):: DNA Learning Center

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Cell – A Movie of RNA Polymerase II Transcription

via cell.com

 

Ribozyme-Catalyzed Transcription of an Active Ribozyme

Abstract

A critical event in the origin of life is thought to have been the emergence of an RNA molecule capable of replicating a primordial RNA “genome.” Here we describe the evolution and engineering of an RNA polymerase ribozyme capable of synthesizing RNAs of up to 95 nucleotides in length. To overcome its sequence dependence, we recombined traits evolved separately in different ribozyme lineages. This yielded a more general polymerase ribozyme that was able to synthesize a wider spectrum of RNA sequences, as we demonstrate by the accurate synthesis of an enzymatically active RNA, a hammerhead endonuclease ribozyme. This recapitulates a central aspect of an RNA-based genetic system: the RNA-catalyzed synthesis of an active ribozyme from an RNA template.

via sciencemag.org

 

 

Ribozyme-catalyzed transcription of an active ribozyme.

Summary

A critical event in the origin of life is thought to have been the emergence of an RNA molecule capable of replicating a primordial RNA “genome.” Here we describe the evolution and engineering of an RNA polymerase ribozyme capable of synthesizing RNAs of up to 95 nucleotides in length. To overcome its sequence dependence, we recombined traits evolved separately in different ribozyme lineages. This yielded a more general polymerase ribozyme that was able to synthesize a wider spectrum of RNA sequences, as we demonstrate by the accurate synthesis of an enzymatically active RNA, a hammerhead endonuclease ribozyme. This recapitulates a central aspect of an RNA-based genetic system: the RNA-catalyzed synthesis of an active ribozyme from an RNA template.

Affiliation

Medical Research Council (MRC) Laboratory of Molecular Biology, Hills Road, Cambridge CB2 0QH, UK.

Journal Details

Name: Science (New York, N.Y.)
ISSN: 1095-9203
Pages: 209-12

Links

 PubMed Articles

 

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