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The RNA Enigma


The RNA Enigma.


A powerful technique to further understanding of RNA

A powerful technique to further understanding of RNA.

Stanford biologists watch RNA fold in real time | Department of Biology


Iron and RNA catalysis

Chemical substitution: On early Earth, iron may have performed magnesium’s RNA folding job

Chemical substitution: On early Earth, iron may have performed magnesium’s #RNA folding job:… #scidaily @MyEN

Jun 1  – Sciencedaily (Reply uRemove Tweet hRetweet òMore

RNA Folding and Catalysis Mediated by Iron (II)


Mg2+ shares a distinctive relationship with RNA, playing important and specific roles in the folding and function of essentially all large RNAs. Here we use theory and experiment to evaluate Fe2+ in the absence of free oxygen as a replacement for Mg2+ in RNA folding and catalysis. We describe both quantum mechanical calculations and experiments that suggest that the roles of Mg2+ in RNA folding and function can indeed be served by Fe2+. The results of quantum mechanical calculations show that the geometry of coordination of Fe2+ by RNA phosphates is similar to that of Mg2+. Chemical footprinting experiments suggest that the conformation of the Tetrahymena thermophila Group I intron P4P6 domain RNA is conserved between complexes with Fe2+ or Mg2+. The catalytic activities of both the L1 ribozyme ligase, obtained previously by in vitro selection in the presence of Mg2+, and the hammerhead ribozyme are enhanced in the presence of Fe2+ compared to Mg2+. All chemical footprinting and ribozyme assays in the presence of Fe2+ were performed under anaerobic conditions. The primary motivation of this work is to understand RNA in plausible early earth conditions. Life originated during the early Archean Eon, characterized by a non-oxidative atmosphere and abundant soluble Fe2+. The combined biochemical and paleogeological data are consistent with a role for Fe2+ in an RNA World. RNA and Fe2+ could, in principle, support an array of RNA structures and catalytic functions more diverse than RNA with Mg2+ alone.

EteRNA on FORA.tv – Open Education and the Future of Science Education

EteRNA on the web


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Chemical Structure of RNA



Evolutionarily young protein helps ancient RNA get into shape



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